LOCUS       EF1a-RLuc-IRES-H        6627 bp    DNA     linear        8-JUL-2013
SOURCE      
  ORGANISM  
            This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     LTR             6393..6627
                     /label=3'\LTR
     intron          291..1455
                     /label=Intron
     LTR             1..181
                     /label=5'LTR
     misc_feature    5714..6310
                     /label=WPRE
     promoter        1698..3087
                     /label=human\EF1a\promoter
     intron          2126..3068
                     /label=intron
     CDS             4679..5704
                     /label=Hyg
     RBS             4071..4655
                     /label=IRES
     CDS             3134..4066
                     /label=Renilla\Luciferase\(RLuc)
BASE COUNT     1552 a      1684 c      1870 g      1521 t 
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga 
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa 
     1681 ttcaaaattt tatcgatgcc tccccgtcac cacccccccc aacccgcccc gaccggagct 
     1741 gagagtaatt catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta 
     1801 aactcccact aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc 
     1861 tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga 
     1921 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc 
     1981 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 
     2041 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 
     2101 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct 
     2161 ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg 
     2221 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt 
     2281 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg 
     2341 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt 
     2401 aaaatttttg atgatatcct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg 
     2461 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg 
     2521 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac 
     2581 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg 
     2641 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc 
     2701 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg 
     2761 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg 
     2821 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta 
     2881 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg 
     2941 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt 
     3001 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc 
     3061 catttcaggt gtcgtgaaaa ctacccctct agataatacg actcactata gggcccgggt 
     3121 tggatccgcc accatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac 
     3181 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta 
     3241 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc 
     3301 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc 
     3361 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga 
     3421 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt 
     3481 tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa 
     3541 gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg 
     3601 gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct 
     3661 tgagaataac ttcttcgtcg agaccatgct cccaagcaag atcatgcgga aactggagcc 
     3721 tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac 
     3781 cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat 
     3841 tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaaga tgttcatcga 
     3901 gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac 
     3961 cgagttcgtg aaggtgaagg gcctccactt cagccaggag gacgctccag atgaaatggg 
     4021 taagtacatc aagagcttcg tggagcgcgt gctgaagaac gagtaagcta gcccctctcc 
     4081 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt 
     4141 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg 
     4201 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg 
     4261 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc 
     4321 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca 
     4381 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag 
     4441 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa 
     4501 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt 
     4561 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt 
     4621 tttcctttga aaaacacgat gataatatgg ccacactaga gatccaccgg tcgccaccat 
     4681 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag 
     4741 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt 
     4801 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg 
     4861 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg 
     4921 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca 
     4981 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc 
     5041 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat 
     5101 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca 
     5161 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct 
     5221 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc 
     5281 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat 
     5341 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg 
     5401 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg 
     5461 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg 
     5521 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc 
     5581 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg 
     5641 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga 
     5701 ataggcggcc gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 
     5761 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 
     5821 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 
     5881 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 
     5941 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 
     6001 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 
     6061 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 
     6121 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 
     6181 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 
     6241 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 
     6301 tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag 
     6361 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 
     6421 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 
     6481 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 
     6541 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 
     6601 tttagtcagt gtggaaaatc tctagca 
//
